>
产品中心 >
Cell_transfection >
Signagen/AAV-Syn-FLPo (AAV Serotype 2)/SL101448/>30 µL (Std Pack)
ProductCategory:Pre-maderecombinantAAV;AAV-Synapsin-FLPo;AAV-Syn-FLPo;AAV2-Synapsin-FLPo;AAV2-Syn-FLPo
Description: FLPoriginallyidentifiedfromSaccharomycescerevisiaeisasite-specificrecombinases(SSR)fromtheλintegrasefamilyandrecognizesdistinct34bpFRTsequence.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.InitialuseofFLPinmammaliancellsrevealedinefficientrecombinaseactivityduetoThermo-instABIlityoftheFLPprotein.Subsequentscreeningforthermo-stablemutantsresultedintheidentificationofFLPewhichwas4-foldincreaseinrecombinationefficiencyat37°C.HowevertherecombinationefficiencyofFLPeincellsremainsverylowbecauseofitsnon-mammalianorigin.
FLPoisamousecodonoptimizedFLPethatgreatlyincreasestheFRTrecombinationefficiencyforbothinvivoandinvitroinmouse. ThisAAV2-Synapsin-FLPopre-made rAAVinSEROtype2(withcapsidfromAAVserotype2and2xITRfromAAVserotype2)will over-expressFLPorecombinaseunderhumansynapsinpromoter(exclusiveneuronalexpression). Readytouseformat.
Titer:>1x1013VG/mL
Size:>30µL(StdPack)
StorageBuffer:PBS
InfectionProtocol:
VectorMap:
